Michael Kallos
Associate Professor, Department of Chemical and Petroleum Engineering
Adjunct Associate Professor, Department of Cell Biology and Anatomy, Faculty of Medicine
- Associate Director Pharmaceutical Production Research Facility (PPRF)
Director, Biomedical Engineering Graduate Program (BME GRAD)
Associate Director, Graduate, Center for Bioengineering Research and Education (CBRE)
- Room: EN B204C
- Telephone: (403) 220-7447
- Fax: (403) 284-4852
- E-mail: mskallos [at] ucalgary [dot] ca
Awards / Accolades:
Profile:
Although born in Cape Town, South Africa, Dr. Kallos is a native to Calgary. He graduated with his PhD in 1999 from the University of Calgary and he is currently working in the area of biochemical and biomedical engineering.
He is the Associate Director of the Pharmaceutical Production Research Facility (PPRF), where he collaborates with Dr. Leo Behie and Dr. Arin Sen. Dr. Kallos is working on projects to generate clinically relevant quantities of embryonic stem cells and their derivatives, including bone and cartilage. He has also been heavily involved in the graduate and undergraduate Biomedical Engineering program at the University of Calgary.
Research Interests:
Tissue engineering, bioreactor design, cell culture, biotechnology, animal cells, neural stem cells, embryonic stem cells, serum-free medium development, chemical reactor kinetics, mass transfer, modeling.
Books:
Embryonic Stem Cells - Differentiation and Pluripotent Alternatives, Michael S. Kallos (Ed.) – InTech Open Access Publisher, Vienna, Austria, ISBN: 978-953-307-632-4 (October 2011) LINK
Embryonic Stem Cells - Basic Biology to Bioengineering, Michael S. Kallos (Ed.) – InTech Open Access Publisher, Vienna, Austria, ISBN: 978-953-307-278-4 (September 2011) LINK
Recent Papers:
2012
Yuan Y, Hunter, CJ, Kallos MS, Sen A; “Microcarrier-Based Expansion of Human Bone Marrow-Derived Mesenchymal Stem Cells in Suspension Culture for Intervertebral Disc Tissue Engineering” Journal of Tissue Engineering and Regenerative Medicine (In Press, Accepted January 2012)
Gareau T, Lara G, Shepherd RD, Taiani J, Krawetz R, Rancourt D, Rinker KD, Kallos MS, “Shear Stress Influences Pluripotency of Murine Embryonic Stem Cells (mESCs) in Suspension Bioreactors” Journal of Tissue Engineering and Regenerative Medicine (In Press, Accepted January 2012)
Kapadia, P. R., Kallos, M. S. and Gates, I. D. (2012), A new reaction model for aquathermolysis of athabasca bitumen. The Canadian Journal of Chemical Engineering. doi: 10.1002/cjce.21662
Van Winkle, A., Gates, I.D., Kallos, M. Mass Transfer Limitations in Embryoid Bodies during Human Embryonic Stem Cell Differentiation. Cells Tissues Organs. Published online January 2012. DOI: 10.1159/000330691
2011
Shafa M, Sjonnesen K, Yamashita A, Liu S, Kallos MS, Rancourt DE, “Expansion and long term maintenance of induced pluripotent stem cells in stirred suspension bioreactors” Journal of Tissue Engineering and Regenerative Medicine (2011 Jul 14) doi: 10.1002/term.450. [Epub ahead of print]
Van Winkle, A.P., Gates, I.D., Kallos, M.S., “Small-Scale Bioreactors for the Culture of Embryonic Stem Cells”, invited Chapter in the Book – Embryonic Stem Cells - Basic Biology to Bioengineering, Michael S. Kallos (Ed.) – InTech Open Access Publisher, Vienna, Austria, ISBN: 978-953-307-278-4 (September 2011) LINK
Hunt, M., Alfred, R., Rancourt, D.E., Gates, I.D., Kallos, M.S., “Bioprocess Considerations for Expansion of Embryonic Stem Cells”, invited Chapter in the Book – Embryonic Stem Cells - Basic Biology to Bioengineering, Michael S. Kallos (Ed.) – InTech Open Access Publisher, Vienna, Austria, ISBN: 978-953-307-278-4 (September 2011) LINK
Kapadia, P.R., Kallos, M.S., and Gates, I.D. Potential for Hydrogen Generation from In Situ Combustion of Athabasca Bitumen. Fuel, 90:2254-2265, 2011
Alfred, R., Fan, J., Boon, K., Krawetz, R., Rancourt, D., and Kallos, MS., “Efficient Suspension Bioreactor Expansion of Murine Embryonic Stem Cells (mESCs) on Microcarriers in Serum-Free Medium”, Biotechnology Progress (Published online March 21, 2011, DOI: 10.1002/btpr.591)
Lee, H., Fisher, S., Kallos, MS., Hunter, C.J., "Optimizing Gelling Parameters of Gellan Gum for Fibrocartilage Tissue Engineering ", Journal of Biomedical Materials Research Part B: Applied Biomaterials, (Accepted February 11, 2011)
Alfred, R., Krawetz, R., Rancourt, D., and Kallos, MS., “Scaled-up Production of Murine Embryonic Stem Cell (mESC) Derived Osteoblasts on Microcarriers in Serum Free Medium”, Biomaterials (Accepted March 29, 2011)
2010
Alfred, R., Gareau, T., Krawetz, R., Rancourt, D., and Kallos, MS., "Serum-Free Scaled up Expansion and Differentiation of Murine Embryonic Stem Cells to Osteoblasts in Suspension Bioreactors", Biotechnology and Bioengineering 106(5), 829-840 (2010). Published online March 26, 2010, DOI 10.1002/bit.22727
Sen A, Kallos MS, Behie LA, "Bioprocess Engineering of Neural Stem Cells", Wiley Encyclopedia of Industrial Biotechnology, Ed MC Flickinger, Publ John Wiley & Sons, ISBN: 978-0-471-79930-6 (2010).
Baghbaderani BA, Sen A, Kallos MS, Behie LA, "Bioengineering Protocols for Neural Precursor Cell Expansion", Chapter in the book Protocols for Neural Cell Culture (4th Edition), Ed L Doering, Publ The Humana Press (4th Edition), Ed L Doering, Series: Springer Protocols Handbooks, ISBN: 978-1-60761-291-9 (2010).
2009
Taiani J, Krawetz RJ, Nieden NZ, Wu YE, Kallos MS, Matyas JR, Rancourt DE, "Reduced Differentiation Efficiency of Murine Embryonic Stem Cells in Stirred Suspension Bioreactors" Stem Cells Dev. 2009 Sep 23. [Epub ahead of print]
Krawetz RJ, Taiani JT, Meng G, Liu S, Li X, Kallos MS, Rancourt DE, "Large-Scale Expansion of Pluripotent Human Embryonic Stem Cells in Stirred Suspension Bioreactors" Tissue Engineering Part C: Methods -Not available-, ahead of print. doi:10.1089/ten.TEC.2009.0228. (Online Ahead of Editing: September 8, 2009).
Baghbaderani BA, Mukhida K, Sen A, Kallos MS, Hong M, Mendez I, Behie LA, " Bioreactor Expansion of Human Neural Precursor Cells in Serum-Free Media Retains Neurogenic Potential", Biotechnology and Bioengineering (accepted October 21, 2009).
Jung S, Sen A, Kallos MS, Behie LA, Rosenberg L, Kutlu B, Goodman N, "Large-Scale Production of Functional Beta Cells from Stem/Progenitor Cells in Suspension Bioreactors", Chapter 29 in the book The Bioartificial Pancreas and Other Biohybrid Therapies, Eds JP Hallé, P de Vos, L Rosenberg, Publ Research Signpost (in press, September, 2009).
2008
Meng GL, zur Nieden NI, Liu SY, Cormier JT, Kallos MS, Rancourt DE, "Properties of murine embryonic stem cells maintained on human foreskin fibroblasts without LIF" Molecular reproduction and development 75(4), 614-22 (2008). (Published Online 20 September 2007: DOI: 10.1002/mrd.20790)
2007
zur Nieden, NI, Cormier, JT, Rancourt, DE, Kallos, MS, "Embryonic Stem Cells Remain Highly Pluripotent Following Long Term Expansion as Aggregates in Suspension Bioreactors", Journal of Biotechnology 129, 421-432 (2007), (Published Online 21 January 2007: doi: 10.1016/j.jbiotec.2007.01.006)
2006
Bodnar, C., Sen A., Kallos, M.S., Behie, L.A., Petropavlovskaia, M., and Rosenberg, L., "Characterization of Human Islet-Like Structures Generated from Pancreatic Progenitor Cells in Culture", Biotechnology and Bioengineering 93(5), 980-988 (2006). (Published Online December 12, 2005: DOI 10.1002/bit.20801).
Chawla, M., Bodnar, C.A., Sen A., Kallos, M.S., and Behie, L.A. "Production of Islet-Like Structures from Neonatal Porcine Pancreatic Tissue in Suspension Bioreactors", Biotechnology Progress 22(2), 561-567 (2006) (Published Online: 11 Mar 2006: DOI: 10.1021/bp050261i).
Gilbertson, J., Sen, A., Behie, L.A., and Kallos, M.S., "Scaled-up Production of Mammalian Neural Stem and Progenitor Cells in Computer-Controlled Suspension Bioreactors" Biotechnology and Bioengineering 94(4) 783-792 (2006). (Published Online: 17 Feb 2006: DOI: 10.1002/bit.20900).
Wang, T.Y., Sen, A., Behie, L.A., and Kallos, M.S., "Dynamic Behaviour of Cells within Neurospheres in Expanding Populations of Neural Precursors" Brain Research 1107, 82-96 (2006). (Published Online 21 July 2006: DOI: 10.1016/j.brainres.2006.05.110)
Cormier, JT, zur Nieden, NI, Rancourt, DE, Kallos, MS, "Expansion of Undifferentiated Murine Embryonic Stem Cells as Aggregates in Suspension Culture Bioreactors" Tissue Eng 12(11) 3233-3245 (2006).