Interim Department Head
PhD - Chemical Engineering,
BSc (with distinction) - Chemical Engineering with Distinction
Dr. Kallos’ laboratory has been a leader in the development of bioprocesses for stem cell expansion and differentiation. They have specific experience with expanding cells as aggregates or adherent cells on microcarriers in stirred suspension bioreactors. His laboratory is a state-of-the-art 400-m2 tissue culture facility (Biosafety Level 2) focusing its research on biomedical and biochemical engineering, and specializing in animal/mammalian tissue culture and large scale expansion of stem/progenitor cells. Dr. Kallos’ lab is equipped with extensive sample analysis capabilities and modern laboratory equipment including a mini-pilot plant which has the capability of monitoring and controlling the reproducible expansion of cells. The lab recently upgraded the bioreactor system to include an array of custom-built mini-bioreactors (10 mL). These enable large numbers of experiments to be performed quickly and more efficiently, before scaling up to larger volumes.
Dr. Kallos’ lab also has extensive analysis capabilities. Analysis equipment includes a HP 1100 Series HPLC and a recently updated BioProfile 100 Plus Analyzer. These machines enable the quick and accurate medium analysis necessary for tissue culture work, medium development and bioreactor scale-up.
Dr. Michael Kallos is a professor in the Department of Chemical and Petroleum Engineering, Schulich School of Engineering, with an adjunct appointment in the Department of Cell Biology and Anatomy in the Faculty of Medicine. He is the Associate Director of the Pharmaceutical Production Research Facility (PPRF), the Director of the Biomedical Engineering (BME) Graduate Program and Associate Director of the Center for Bioengineering Research and Education (CBRE). Dr. Kallos runs an NSERC funded bioengineering lab investigating the development of scaled-up expansion and differentiation bioprocesses for adult/ embryonic stem cells, and he is also a member of the McCaig Institute for Bone and Joint Health in the Faculty of Medicine. He is a Principal Investigator in the Stem Cell Network, where he has served on the Training Committee, as well as the Steering Committees for the 2007 and 2010 AGM. Dr. Kallos has received a number of awards, including an ASTech (Alberta Science and Technology Leadership Foundation) Leader of Tomorrow Award (2002), numerous teaching awards from peers and students (including “Professor of the Year” and “Outstanding Excellence in Teaching”), and other honors including being named as one of Calgary’s Top 40 Under 40 (Avenue Magazine, 2009). Dr. Kallos has significant service contributions including being involved in organizing several local, national and international conferences, and has received a Faculty of Engineering Service Award in 2005 and again in 2013 (Schulich School of Engineering). He has received funding from several Canadian and international granting and funding agencies including NSERC, CIHR, NIH, CFI, ASRIP, JDRF and the Bill and Melinda Gates Foundation.
Embryonic Stem Cells - Differentiation and Pluripotent Alternatives, Michael S. Kallos (Ed.) – InTech Open Access Publisher, Vienna, Austria, ISBN: 978-953-307-632-4 (October 2011) LINK
Embryonic Stem Cells - Basic Biology to Bioengineering, Michael S. Kallos (Ed.) – InTech Open Access Publisher, Vienna, Austria, ISBN: 978-953-307-278-4 (September 2011) LINK
Anjum F, Lienemann PS, Metzger S, Biernaskie J, Kallos MS, Ehrbar M, (2016) Enzyme responsive GAG-based natural-synthetic hybrid hydrogel for tunable growth factor delivery and stem cell differentiation. Biomaterials. 87:104-17.
Shakhbazau A, Mirfeizi L, Walsh T, Wobma HM, Kumar R, Singh B, Kallos MS, Midha R. (2016) Inter-microcarrier transfer and phenotypics tability of stem cell-derived Schwann cells in stirred suspension bioreactorculture. Biotechnology and Bioengineering 113(2), 393-402.
Hunt, M.M., Meng, G., Rancourt, D.E., Gates, I.D., Kallos, M.S. (2014) Factorial Experimental Design for the Culture of Human Embryonic Stem Cells as Aggregates in Stirred Suspension Bioreactors Reveals the Potential for Interaction Effects Between Bioprocess Parameters. Tissue Engineering, Part C, 1(1) 76-89.
Gareau T, Lara G, Shepherd RD, Taiani J, Krawetz R, Rancourt D, Rinker KD, Kallos MS, (2014) Shear Stress Influences Pluripotency of Murine Embryonic Stem Cells (mESCs) in Suspension Bioreactors. Journal of Tissue Engineering and Regenerative Medicine 8(4):268-78.
Yuan Y, Hunter, CJ, Kallos MS, Sen A; (2014) Improved expansion of human bone marrow-derived mesenchymal stem cells in microcarrier-based suspension culture. Journal of Tissue Engineering and Regenerative Medicine 8(3):210-25.
Lara G, Hazenbiller O, Gareau T, Shepherd RD, Kallos MS, Rancourt D, Rinker KD, (2012) “Fluid Flow Induction of Murine Embryonic Stem Cell Pluripotency Gene Expression in the Absence of LIF” Cell. Mol. Bioeng. 6(3), 335-345.
Hunt, M.M., Meng, G., Rancourt, D.E., Gates, I.D., Kallos, M.S. Factorial Experimental Design for the Culture of Human Embryonic Stem Cells as Aggregates in Stirred Suspension Bioreactors Reveals the Potential for Interaction Effects Between Bioprocess Parameters. Tissue Engineering, Part C, May 2013. Posted online on May 14, 2013. (doi:10.1089/ten.TEC.2013.0040)
Gates, Ian D., Marya Cokar, and Michael S. Kallos, 2013, Fundamentals of heat transport at the edge of steam chambers in cyclic steam stimulation and steam-assisted gravity drainage, in F. J. Hein, D. Leckie, S. Larter, and J. R. Suter, eds., Heavy-oil and oil-sand petroleum systems in Alberta and beyond: AAPG Studies in Geology 64, p. 625–638.
Cokar, M., Ford, B., Gieg, L., Kallos, M.S., and Gates, I.D. "Reactive Reservoir Simulation of Biogenic Shallow Shale Gas Systems Enabled by Experimentally-Determined Methane Generation Rates". Accepted. Energy & Fuels, April 2013.
Kapadia, P.R., Kallos, M.S., and Gates, I.D. "A New Kinetic Model for Pyrolysis of Athabasca Bitumen". Canadian Journal of Chemical Engineering, 91(5):889-901, 2013.
Hunt, M., Meng, G., Rancourt, D.E., Gates, I.D., Kallos, M.S., "Image Analysis Method for Evaluating Heterogeneous Growth and Differentiation of Embryonic Stem Cell Cultures", Chapter 10 in Developments in Biotechnology and Bioprocessing. 2013, American Chemical Society. p. 165-181. Chapter DOI: 10.1021/bk-2013-1125.ch010, ACS Symposium Series, Vol. 1125,ISBN13: 9780841229105 eISBN: 9780841229112, Publication Date (Web): March 8, 2013
Kapadia, P., Wang, J., Kallos, M.S., and Gates, I.D. "Practical Process Design for In Situ Gasification of Bitumen" (2013). Applied Energy, 107, pp. 281-296.
Cokar M, Kallos MS, Gates ID “A New Thermogeomechanical Theory for Gravity Drainage in SAGD”, SPE Journal, (Accepted December 4, 2012 manuscript ID is SJ-0212-0028).
Ezeuko, C, Wang, Jacky, Kallos, MS, Gates, ID., (2012) “Towards the Development of Bitumen Carbonates: An Integrated Analysis of Grosmont Steam Pilots”, Oil & Gas Science and Technology - Revue d'IFP Energies Nouvelles (Accepted Nov 23, 2012)
Cokar M, Kallos MS, Gates ID “Reservoir Simulation of Steam Fracturing in Early Cycle Cyclic Steam Stimulation”, SPE Reservoir Evaluation and Engineering – Reservoir Engineering, (SPE paper number SPE-129686-PA – Accepted Oct. 4, 2012).
Shakhbazau A, Shcharbin D, Bryszewska M, Kumar R, Wobma, HM, Kallos MS, Goncharova N, Seviaryn I, Kosmacheva S, Potapnev M, Midha R. (2012) “Non-viral engineering of skin precursor-derived Schwann cells for enhanced NT-3 production in adherent and microcarrier culture”, Current Medicinal Chemistry 19(32) p5572-5579 (8)
Kapadia, P.R., Wang, J., Kallos, M.S., and Gates, I.D., “New Thermal-Reactive Reservoir Engineering Model Predicts Hydrogen Sulfide Generation in Steam Assisted Gravity Drainage”, J. Petrol.Science and Engineering Volumes 94–95, September 2012, 100-111.
Cokar M, Ford B, Kallos MS, Gates ID; “New Gas Material Balance to Quantify Biogenic Gas Generation Rates from Shallow Organic-Matter-Rich Shales” Fuel Available online 4 July 2012, ISSN 0016-2361, 10.1016/j.fuel.2012.06.054.
Yuan Y, Hunter, CJ, Kallos MS, Sen A; (2012) “Microcarrier-Based Expansion of Human Bone Marrow-Derived Mesenchymal Stem Cells in Suspension Culture for Intervertebral Disc Tissue Engineering” Journal of Tissue Engineering and Regenerative Medicine doi: 10.1002/term.1515
Gareau T, Lara G, Shepherd RD, Taiani J, Krawetz R, Rancourt D, Rinker KD, Kallos MS, (2012) “Shear Stress Influences Pluripotency of Murine Embryonic Stem Cells (mESCs) in Suspension Bioreactors” Journal of Tissue Engineering and Regenerative Medicine (doi: 10.1002/term.1518)
Kapadia, P. R., Kallos, M. S. and Gates, I. D. (2012), A new reaction model for aquathermolysis of athabasca bitumen. The Canadian Journal of Chemical Engineering.doi: 10.1002/cjce.21662
Van Winkle, A., Gates, I.D., Kallos, M. Mass Transfer Limitations in Embryoid Bodies during Human Embryonic Stem Cell Differentiation. Cells Tissues Organs. 2012;196:34-47 (DOI: 10.1159/000330691
Shafa M, Sjonnesen K, Yamashita A, Liu S, Kallos MS, Rancourt DE, “Expansion and long term maintenance of induced pluripotent stem cells in stirred suspension bioreactors” Journal of Tissue Engineering and Regenerative Medicine (2011 Jul 14) doi: 10.1002/term.450. [Epub ahead of print]
Van Winkle, A.P., Gates, I.D., Kallos, M.S., “Small-Scale Bioreactors for the Culture of Embryonic Stem Cells”, invited Chapter in the Book – Embryonic Stem Cells - Basic Biology to Bioengineering, Michael S. Kallos (Ed.) – InTech Open Access Publisher, Vienna, Austria, ISBN: 978-953-307-278-4 (September 2011) LINK
Hunt, M., Alfred, R., Rancourt, D.E., Gates, I.D., Kallos, M.S., “Bioprocess Considerations for Expansion of Embryonic Stem Cells”, invited Chapter in the Book – Embryonic Stem Cells - Basic Biology to Bioengineering, Michael S. Kallos (Ed.) – InTech Open Access Publisher, Vienna, Austria, ISBN: 978-953-307-278-4 (September 2011) LINK
Kapadia, P.R., Kallos, M.S., and Gates, I.D. Potential for Hydrogen Generation from In Situ Combustion of Athabasca Bitumen. Fuel, 90:2254-2265, 2011
Alfred, R., Fan, J., Boon, K., Krawetz, R., Rancourt, D., and Kallos, MS., “Efficient Suspension Bioreactor Expansion of Murine Embryonic Stem Cells (mESCs) on Microcarriers in Serum-Free Medium”, Biotechnology Progress (Published online March 21, 2011, DOI: 10.1002/btpr.591)
Lee, H., Fisher, S., Kallos, MS., Hunter, C.J., "Optimizing Gelling Parameters of Gellan Gum for Fibrocartilage Tissue Engineering ", Journal of Biomedical Materials Research Part B: Applied Biomaterials, (Accepted February 11, 2011)
Alfred, R., Krawetz, R., Rancourt, D., and Kallos, MS., “Scaled-up Production of Murine Embryonic Stem Cell (mESC) Derived Osteoblasts on Microcarriers in Serum Free Medium”, Biomaterials (Accepted March 29, 2011)
Alfred, R., Gareau, T., Krawetz, R., Rancourt, D., and Kallos, MS., "Serum-Free Scaled up Expansion and Differentiation of Murine Embryonic Stem Cells to Osteoblasts in Suspension Bioreactors", Biotechnology and Bioengineering 106(5), 829-840 (2010). Published online March 26, 2010, DOI 10.1002/bit.22727
Sen A, Kallos MS, Behie LA, "Bioprocess Engineering of Neural Stem Cells", Wiley Encyclopedia of Industrial Biotechnology, Ed MC Flickinger, Publ John Wiley & Sons, ISBN: 978-0-471-79930-6 (2010).
Baghbaderani BA, Sen A, Kallos MS, Behie LA, "Bioengineering Protocols for Neural Precursor Cell Expansion", Chapter in the book Protocols for Neural Cell Culture (4th Edition), Ed L Doering, Publ The Humana Press (4th Edition), Ed L Doering, Series: Springer Protocols Handbooks, ISBN: 978-1-60761-291-9 (2010).
Taiani J, Krawetz RJ, Nieden NZ, Wu YE, Kallos MS, Matyas JR, Rancourt DE, "Reduced Differentiation Efficiency of Murine Embryonic Stem Cells in Stirred Suspension Bioreactors" Stem Cells Dev. 2009 Sep 23. [Epub ahead of print]
Krawetz RJ, Taiani JT, Meng G, Liu S, Li X, Kallos MS, Rancourt DE, "Large-Scale Expansion of Pluripotent Human Embryonic Stem Cells in Stirred Suspension Bioreactors" Tissue Engineering Part C: Methods -Not available-, ahead of print. doi:10.1089/ten.TEC.2009.0228. (Online Ahead of Editing: September 8, 2009).
Baghbaderani BA, Mukhida K, Sen A, Kallos MS, Hong M, Mendez I, Behie LA, " Bioreactor Expansion of Human Neural Precursor Cells in Serum-Free Media Retains Neurogenic Potential", Biotechnology and Bioengineering (accepted October 21, 2009).
Jung S, Sen A, Kallos MS, Behie LA, Rosenberg L, Kutlu B, Goodman N, "Large-Scale Production of Functional Beta Cells from Stem/Progenitor Cells in Suspension Bioreactors", Chapter 29 in the book The Bioartificial Pancreas and Other Biohybrid Therapies, Eds JP Hallé, P de Vos, L Rosenberg, Publ Research Signpost (in press, September, 2009).
Meng GL, zur Nieden NI, Liu SY, Cormier JT, Kallos MS, Rancourt DE, "Properties of murine embryonic stem cells maintained on human foreskin fibroblasts without LIF" Molecular reproduction and development 75(4), 614-22 (2008). (Published Online 20 September 2007: DOI: 10.1002/mrd.20790)
zur Nieden, NI, Cormier, JT, Rancourt, DE, Kallos, MS, "Embryonic Stem Cells Remain Highly Pluripotent Following Long Term Expansion as Aggregates in Suspension Bioreactors", Journal of Biotechnology 129, 421-432 (2007), (Published Online 21 January 2007: doi: 10.1016/j.jbiotec.2007.01.006)
Bodnar, C., Sen A., Kallos, M.S., Behie, L.A., Petropavlovskaia, M., and Rosenberg, L., "Characterization of Human Islet-Like Structures Generated from Pancreatic Progenitor Cells in Culture", Biotechnology and Bioengineering 93(5), 980-988 (2006). (Published Online December 12, 2005: DOI 10.1002/bit.20801).
Chawla, M., Bodnar, C.A., Sen A., Kallos, M.S., and Behie, L.A. "Production of Islet-Like Structures from Neonatal Porcine Pancreatic Tissue in Suspension Bioreactors", Biotechnology Progress 22(2), 561-567 (2006) (Published Online: 11 Mar 2006: DOI: 10.1021/bp050261i).
Gilbertson, J., Sen, A., Behie, L.A., and Kallos, M.S., "Scaled-up Production of Mammalian Neural Stem and Progenitor Cells in Computer-Controlled Suspension Bioreactors" Biotechnology and Bioengineering 94(4) 783-792 (2006). (Published Online: 17 Feb 2006: DOI: 10.1002/bit.20900).
Wang, T.Y., Sen, A., Behie, L.A., and Kallos, M.S., "Dynamic Behaviour of Cells within Neurospheres in Expanding Populations of Neural Precursors" Brain Research 1107, 82-96 (2006). (Published Online 21 July 2006: DOI: 10.1016/j.brainres.2006.05.110)
Cormier, JT, zur Nieden, NI, Rancourt, DE, Kallos, MS, "Expansion of Undifferentiated Murine Embryonic Stem Cells as Aggregates in Suspension Culture Bioreactors" Tissue Eng 12(11) 3233-3245 (2006).